WebMay 12, 2024 · CYP76F112 kinetic parameters were determined by incubating 0.54 pmol of functional P450 into 0.1 M NaPi buffer, pH 7.0 containing an excess of NADPH and 10 to … WebMar 18, 2024 · CYP76F112 was found to convert demethylsuberosin into marmesin with a very high affinity. This atypical cyclization reaction represents a key step within the …
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WebIsolation and functional characterization of a cDNA coding a hydroxycinnamoyltransferase involved in phenylpropanoid biosynthesis in Cynara cardunculus L by Cinzia Comino ( ) 1 edition published in 2007 in English and held by 2 WorldCat member libraries worldwide WebIt has been reported that the furan or pyran ring was formed by the cyclization of isopentenyl and ortho -hydroxyl under the catalysis of cytochrome P450s. [ref], [ref] To identify the P450s responsible for the formation of furan and pyran rings in moracins, the same method for PTs screening was used for P450s, and 17 candidate unigenes with a … details of the gps m code signal
Challenges in the Heterologous Production of Furanocoumarins in ...
WebK25522 CYP76F112; marmesin synthase Enzymes [BR:ko01000] 1. Oxidoreductases 1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen 1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor 1.14.14.66 marmesin synthase K25522 CYP76F112; marmesin synthase WebFeb 28, 2024 · The characterization of CYP76F112 as the first known marmesin synthase opens new prospects for the use of the furanocoumarin pathway, and supports the multiple acquisition of furanicellin in angiosperms by convergent evolution, and opens new perspectives regarding the ability of cytochromes P450 to evolve new functions related to … WebCYP76F112 was found to convert demethylsuberosin into marmesin with a very high affinity. This atypical cyclisation reaction represents a key step within the polyphenol biosynthesis pathway. CYP76F112 evolutionary patterns suggests that the marmesin synthase activity appeared recently in the Moraceae family, through a lineage-specific expansion ... chung\u0027s braintree