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Klf4 recombinant protein

WebOur results demonstrated for the first time that TAT- and Penetratin-linked Klf4 proteins can effectively replace viral Klf4 in reprogramming fibroblasts, and provided a valuable … WebMar 6, 2024 · Active Recombinant human KLF4 protein is an Escherichia coli Full length protein 1 to 479 aa range, > 75% purity and validated in FuncS, SDS-PAGE. The in vitro function was tested using specific DNA…

Recombinant Human KLF4 protein (ab204759) Abcam

WebKLF4 is a member of the erythroid Kruppel-like factor (EKLF) multigene family that is highly expressed in the differentiating layers of the epidermis (1, 2). KLF4 plays a critical role in the differentiation of epithelial cells and is essential for normal gastric homeostasis (2,3). WebJul 1, 2024 · Abstract and Figures The four transcription factors Oct4, Sox2, Klf4 and c-Myc are highly expressed in embryonic stem cells (ESC) and their overexpression can induce pluripotency, an ability to... hugh johnson wine guide 2023 https://elitefitnessbemidji.com

Human Recombinant KLF4-TAT (from HEK293 Cells) VWR

WebSoluble expression of recomb inant cMyc, Klf4, Oct4, and Sox2 proteins in bacteria and transduction into living cells Guo-Dan Liu 1, Shi-Feng Zhou2, Xu-Chen Ding1, Chun-Lai Fang1, Shu-Yong Mi, Xiang-Chun Gao, ... The recombinant proteins were purified by Ni-NTA resin and identified by Western blot. The transduction WebNov 24, 2024 · Another improvement was the discovery of a growth-factor-free culture system by Yasuda et al. Yasuda with coworkers prepared a chemically defined culture medium using only three chemical compounds, no growth factors, and a minimum of recombinant proteins compared to other commercially available culture media. iPSCs … WebAug 19, 2024 · To purify KLF4 and KLF4 K409Q recombinant proteins for footprinting assay, we cloned the coding region of C-terminal 105 aa coding DBD of KLF4 and KLF4 K409Q into pMAL-c6T vector (New England Biolabs, N0378S) and expressed it in the BL21 bacterial strain. The recombinant proteins were purified using amylose magnetic beads (New … hugh johnson wine writer

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Category:Recombinant Human KLF4 Protein (H00009314-P01): …

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Klf4 recombinant protein

Human KLF4-TAT Recombinant Protein Lyophilized

WebKLF4 (EZF, GKLF) protein expression summary. We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. ... Enhanced - Recombinant expression i Single band corresponding to the predicted size in kDa (+/-20%). Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 ... WebSr. Director of R&D, Protein Biochemistry & Neuroscience. • Collaborated with CROs and academic collaborators to design and execute studies to …

Klf4 recombinant protein

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WebRecombinant Human KLF4-TAT is a 483 amino acid protein, including a 13-residue C-terminal TAT peptide, with a calculated molecular weight of 51.7 kDa. PeproTech’s … WebThe Structure and function of KLF4 . Figure 2. The structure of KLF4. The human KLF4 gene is located on chromosome 9q31 and is a single-copy gene located in the minus strand, covering a 6.3 kb gene segment with five exons. Its encoded protein contains 513 amino acids with a molecular mass of approximately 55 kDa.

WebProtein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors into primary, as well as transformed, cells. Recombinant Human KLF4-TAT is a 483 amino acid protein, including a 13-residue C-terminal TAT peptide, with a calculated molecular weight of 51.7 kDa. WebKlf4 encodes Krueppel-like factor 4 (Klf4), a zinc finger transcription factor that is evolutionarily conserved from flies to humans. Klf4 is reported to be expressed in the …

WebMar 28, 2024 · Klf4 protein was then incubated with recombinant active ERK1 (Millipore) and 2 mM ATP at 30 °C for 30 min. ... CTTG-3′. Recombinant GST-Klf4 and mutants were expressed by E.coli and purified as ... WebKLF4 is a member of the Kruppel-like factor (KLF) family of zinc finger transcription factors. Recombinant human KLF4-TAT is a 51.7 kDa protein containing 483 amino acid residues, …

WebThe staining of the antibody is evaluated by Western blot through analyses of samples from cell lysates with and without recombinant expression of the target protein. The results …

WebRecombinant Human KLF4-TAT is a 483 amino acid protein, including a 13-residue C-terminal TAT peptide, with a calculated molecular weight of 51.7kDa. Recombinant … holiday inn express fishersWebKLF4 is a member of the erythroid Kruppel-like factor (EKLF) multigene family that is highly expressed in the differentiating layers of the epidermis (1, 2). KLF4 plays a critical role in the differentiation of epithelial cells and … holiday inn express fisherWebUseful information about the protein provided by UniProt. Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the prom oter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. hugh johns seattleWebJan 24, 2014 · The transcription factor, Krüppel-like factor 4 (Klf4) plays a crucial role in generating induced pluripotent stem cells (iPSCs). As the ubiquitination and degradation of the Klf4 protein have been suggested to play an important role in its function, the identification of specific lysine sites that are responsible for protein degradation is of … holiday inn express flagstaffWebThe product of this gene inhibits renal tubular phosphate transport. This gene was identified by its mutations associated with autosomal dominant hypophosphatemic rickets, an inherited phosphate wasting disorder. Abnormally high level expression of this gene was found in oncogenic hypophosphatemic osteomalacia, a phenotypically similar disease ... holiday inn express flagstaff an ihg hotelWebMar 21, 2024 · KLF4 (KLF Transcription Factor 4) is a Protein Coding gene. Diseases associated with KLF4 include Epilepsy, Idiopathic Generalized 15 and Familial Adenomatous Polyposis . Among its related pathways are … holiday inn express flagstaff areaWebKruppel like factor 4 (KLF4) protein is encoded by the KLF4 gene located on chromosome 9 in humans. It is also known as gut-enriched Kruppel-like factor (GKLF) or epithelial zinc … holiday inn express fisherman san francisco